Bromodeoxyuridine (BrdU) from BioLegend

Product Specs
ItemBromodeoxyuridine (BrdU)
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number423401
Quantity25 mg
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
Concentration10.00 mg/mL
FormatThis product consists of 5 vials. Each vial contains 5 mg/500 µl of BrdU in an aqueous solution

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Transglutaminase 2 Prevents Premature Senescence and Promotes Osteoblastic Differentiation of Mesenchymal Stem Cells through NRF2 ActivationStem Cells InternationalOctober 20, 2023Soo-Jin Lee, Ji-Woong Shin, Mee-Ae Kwon, Ki Baek Lee, Hyo-Jun Kim, Jin-Haeng Lee, Heun-Soo Kang, Jong Kwan Jun, Sung-Yup Cho, In-Gyu KimIncorporation Assay For the BrdU incorporation assay, cells were incubated with 10 μM BrdU (Cat. No. 423401; Biolegend, USA) for indicated times. After fixing the cells with 70% ethanol, cells were permeabilized

(2) RAGE inhibitor TTP488 (Azeliragon) suppresses metastasis in triple-negative breast cancernpj Breast CancerJuly 13, 2023Melinda Magna, Gyong Ha Hwang, Alec McIntosh, Katherine Drews-Elger, Masaru Takabatake, Adam Ikeda, Barbara J. Mera, Taekyoung Kwak, Philip Miller, Marc E. Lippman, et al.µM for 48 h. During the final hour of culture, cells were pulsed with 10 µM BrdU (BioLegend Catalog# 423401), collected by trypsinization, fixed in iced-cold 70% ethanol, and stored at −20C for at least 2 h.

(3) H3K27me3 Demethylase UTX Restrains Plasma Cell FormationThe Journal of ImmunologyApril 15, 2022Anna K. Kania, Madeline J. Price, Lou-Ella George-Alexander, Dillon G. Patterson, Sakeenah L. Hicks, Christopher D. Scharer, Jeremy M. Bossone hour before analysis, the CD45.1 µMT host mice were injected with 0.8 mg bromodeoxyuridine (BrdU; 423401; Biolegend). All animals were housed by the Emory Division of Animal Resources, and all protocols were

(4) Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregationDevelopmental cellDecember 20, 2021Merta H, Carrasquillo Rodríguez JW, Anjur-Dietrich MI, Vitale T, Granade ME, Harris TE, Needleman DJ, Bahmanyar S.(EdU),Lumiprobe,Cat#10540 Sulfo-Cyanine 5 Azide,Lumiprobe,Cat#A3330 Bromodeoxyuridine (BrdU),BioLegend,Cat#423401 Sodium tetraborate decahydrate,Sigma,Cat#S9640 RNAse A,Sigma,Cat#AB12023-00100 Propidium Iodide,BioLegend,Cat#421301 Thymidine,Sigma,T1895 2’ deoxycytidine

(5) An IRF4–MYC–mTORC1 Integrated Pathway Controls Cell Growth and the Proliferative Capacity of Activated B Cells during B Cell Differentiation In VivoThe Journal of ImmunologyOctober 1, 2021Dillon G. Patterson, Anna K. Kania, Madeline J. Price, James R. Rose, Christopher D. Scharer, Jeremy M. Bossintracellular staining In some adoptive transfers, hosts were injected with 800 μg BrdU (Biolegend; 423401) intravenously 1 h prior to euthanasia. For some ex vivo experiments, 5 μg BrdU/mL of culture was added

(6) Neuronal metabotropic glutamate receptor 8 protects against neurodegeneration in CNS inflammationThe Journal of experimental medicineMay 3, 2021Woo MS, Ufer F, Rothammer N, Di Liberto G, Binkle L, Haferkamp U, Sonner JK, Engler JB, Hornig S, Bauer S, et al.positive control. During the last 16 h of culture, cells were pulsed with 1 µg/ml BrdU (catalog no. 423401). Single-cell suspensions were stained for surface antigens in the presence of TruStain Fc receptor

(7) The Hepatic Microenvironment Uniquely Protects Leukemia Cells through Induction of Growth and Survival Pathways Mediated by LIPGCancer discoveryFebruary 1, 2021Ye H, Minhajuddin M, Krug A, Pei S, Chou CH, Culp-Hill R, Ponder J, De Bloois E, Schniedewind B, Amaya ML, et al.APC/Cyanine7 anti-mouse CD127 (IL-7Rα) (135039), APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1) (108125) and BrdU (423401) are purchased from Biolegend. APC Annexin V (550475), PE Rat anti-Mouse CD34 (551387) are purchased

(8) Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regenerationNature CommunicationsDecember 1, 2020Hirohito Abo et al.plus Cyanine 3, and TSA plus Cyanine 5 (PerkinElmer) were used for detection. For BrdU staining, BrdU (423401, Biolegend) was dissolved in PBS to 5 mg/mL and 200 μL was injected intraperitoneally 6 h before euthanization

(9) Tuft-Cell-Derived Leukotrienes Drive Rapid Anti-helminth Immunity in the Small Intestine but Are Dispensable for Anti-protist ImmunityImmunityMarch 17, 2020McGinty JW, Ting HA, Billipp TE, Nadjsombati MS, Khan DM, Barrett NA, Liang HE, Matsumoto I, von Moltke J.20410 Leukotriene B4,Cayman Chemical,Cat# 20110 Montelukast,Cayman Chemical,Cat# 10008318 BrdU,BioLegend,Cat# 423401 Sodium succinate hexahydrate,Alfa Aesar,Cat#41983 Critical Commercial Assays,Critical Commercial Assays,Critical

(10) Permanent neuroglial remodeling of the retina following infiltration of CSF1R inhibition-resistant peripheral monocytesProceedings of the National Academy of Sciences of the United States of AmericaNovember 27, 2018Paschalis EI, Lei F, Zhou C, Kapoulea V, Dana R, Chodosh J, Vavvas DG, Dohlman CH.m-CSF (no. 315-02; PeproTech) and 10 ng/mL IL-4 (no. 214-14; PeproTech) in the presence of BrdU (no. 423401; BioLegend) for 5 d. At the end of coculture (5 d), cells were trypsinized, fixed with 4% PFA, permeabilized

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Bromodeoxyuridine (BrdU) from BioLegend

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Bromodeoxyuridine (BrdU) from BioLegend

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